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. 2012 Jun 26;22(10):1289–1301. doi: 10.1093/glycob/cws103

Fig. 3.

Fig. 3.

Quantification of ganglioside terminal sialylation based on TLC densitometry. Gangliosides of wild-type (wt) and St3gal mutant mice were resolved by TLC, stained with resorcinol, scanned and the bands quantified by densitometry. Since resorcinol staining intensity is proportional to sialic acid content, each ganglioside band density was divided by the number of sialic acids on that ganglioside species. The sum of gangliosides with a NeuAc bound α2-3 to the terminal galactose (GD1a + GT1b + GT1b-OAc + GQ1b) was divided by the total of all ganglioside species to give the percent terminal sialylation. Values are the mean ± SEM for 2–14 individual mice of that genotype. *P << 0.001 compared with wild type.