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. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: Nature. 2010 Mar 28;464(7291):1058–1061. doi: 10.1038/nature08871

Figure 2. Down regulation of cFLIP by APC/β-catenin-mediated activation of c-Myc and modulation of TRAIL receptor expression by RAc contribute to the activation of TRAIL signaling.

Figure 2

(a) NCM356 cells were transfected with either β-catenin or c-Myc and treated with TRAIL, RAc, or both. Cleavage of caspase 8 was detected by Western blotting. (b) NCM356 cells were transfected with Ns-siRNA, APC-siRNA (GFP tagged), or APC-siRNA and c-Myc-siRNA. The transfected cells were treated with RAc and TRAIL, and photographs were taken. Expression of APC, c-Myc, and caspase 8 was detected by Western blotting. (c) NCM356 cells were transfected with c-Myc, cFLIP-siRNA, c-Myc and cFLIP, c-Myc and Bcl-2, or c-Myc and Bcl-XL. The transfected cells were treated with RAc and TRAIL. Expression of c-Myc, cFLIP, Bcl-2, Bcl-XL, and caspase 8 was detected by Western blotting. (d) NCM356 cells were treated with RAc for 48 h, and expression of DR4, DR5, DcR1, and DcR2 was detected by Western blotting. (e) NCM356 cells were transfected with either APC-siRNA or APC-siRNA and DR5 or were pretreated with an anti-DcR1 antibody after APC-siRNA transfection. Nonspecific Ns-siRNA was used as control. Transfected cells were treated with TRAIL alone or with TRAIL and RAc, as indicated. Cleavage of caspase 8 was detected by Western blotting.