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PAPS is biosynthesized in the cytosol of an eukaryotic cell and used there by cytosolic STs to transfer sulfo groups (red) to small endogenous and exogenous compounds, for example steroids. PAPS is also transported to the Golgi where it is used by membrane STs to transfer sulfo groups (red) to hydrophilic molecules, for example carbohydrates. STs in the Golgi consist of a cytosolic tail, transmembrane (TM) domain, and catalytic domain
