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. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: Anal Bioanal Chem. 2012 Apr 12;403(6):1491–1500. doi: 10.1007/s00216-012-5944-4

Scheme 2.

Scheme 2

2-O-ST sulfonates N-sulfoheparosan and CDSNS heparin. The polysaccharide product can be quantified and then treated with heparin lyase to afford disaccharides for characterization against disaccharide standards, using HPLC with scintillation detection to establish the position of the added sulfo group