Table 2.

Comparison of ST assay methods

Assay Method	Benefits	Limitations
Radiometric	Highly sensitive, fast, specific	Hazardous, expensive, discontinuous
Photometric	Fast, inexpensive, continuous, high-throughput capable, kinetic constants easily measurable, requires few sample-processing steps, accurate	Requires chromogenic substrate, relatively less sensitive
Fluorimetric	Highly sensitive, fast, continuous, inexpensive, high-throughput capable, kinetic constants easily measurable, specific, requires few sample-processing steps, accurate	Requires fluorogenic substrate
Mass spectrometric	Highly specific, highly sensitive, highly accurate	Requires expensive equipment, discontinuous, requires specialized skills