FIG. 6.

Rapamycin/IL-2 combination therapy increased eosinophils and sIL-2RA while decreasing serum TGF-β. A: The frequency of eosinophils in whole blood was determined in a standard hematologic assay and is shown for each subject over time. B: Serum analytes were measured using a multiplex Searchlight chemiluminiscent assay. Averages of duplicates for all subjects are shown for day 0 and 84, and subjects 1, 2, 3, 5, and 7 are shown for time points between days 14 and 70. Of the 22 cytokines and cytokine receptors tested, data are shown where statistical differences were observed. Measures are shown for each subject over time, and statistical significance was determined by a one-way ANOVA, noted in the graph. Significance from time 0 to each time point was determined using a paired Student t test and is indicated with an asterisk. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.