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. 2012 Aug 17;61(9):2320–2329. doi: 10.2337/db11-1179

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — RT-PCR gene expression of different PLA₂ enzymes and protein expression of pla2g2a in rat adipose tissue. A: Quantitative comparison of mRNA expression level of PLA₂ enzymes in adipose tissue from rats fed CS vs. HCHF diets for 16 weeks. Values of PLA₂ mRNA levels were normalized relative to the housekeeping gene 18S rRNA. B: Immunoblot of pla2g2a protein levels in adipose tissue with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) used as a loading control. Each lane represents adipose tissue homogenate from a single rat. Optical density of protein bands was determined using ImageJ software. Serum (C) and adipose (D) homogenate concentrations of PGE₂ from CS vs. HCHF fed rats. Error bars represent means ± SEM (n = 3–5 animals). *P < 0.05, **P < 0.01, ***P < 0.001.