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. 2012 Aug 17;61(9):2320–2329. doi: 10.2337/db11-1179

FIG. 5.

FIG. 5.

In vivo responses of KH064 (5 mg/kg/day p.o., weeks 8–16) on the regulation of metabolic parameters that were elevated in rats fed HCHF vs. CS diets for 16 weeks. A: Immunoblot of phospho-HSL (Ser563) in adipose tissue with total HSL as the loading control. Optical density of protein bands was determined using ImageJ software. B: RT-PCR gene expression of genes in adipose tissue of rats fed CS and HCHF diets with and without KH064 treatment. C: Plasma concentrations for lipids nonesterified fatty acids (NEFA), triglycerides, and total cholesterol in rats of different groups (n = 5–10). D: Liver total lipid content in rats of different groups (n = 5). E: Plasma liver enzymes in rats of different groups (n = 5–10). F: Total soluble fecal fat in rats of different groups (n = 5). RT-PCR gene expression of different metabolic genes in liver (G) and skeletal muscle (H). Values of mRNA levels were normalized relative to 18S rRNA. Error bars represent means ± SEM (n = 3–5 animals). *P < 0.05, **P < 0.01, ***P < 0.001.