FIG. 5.

Characterization of human dendritic cells and the expression level of IL-17 in purified T cells as a function of the BMI. FACS analyses were performed on total SVF (patient age = 44.6 ± 2.5 years, BMI ranging from 19.5 to 38 kg/m², n = 20 to 24). A: Cell number of CD1c⁺ cells per gram of AT (P < 0.05, Spearman r = 0.3826, n = 24). B: Cell number of CD1c⁺ CD11c⁺ cells per gram of AT (P < 0.05, Spearman r = 0.4947, n = 20). C: Cell number of CD83⁺ cells per gram of AT (P < 0.05, Spearman r = 0.3870, n = 21). D: The gene expression level of IL-17 was evaluated by real-time PCR on immunoselected CD3⁺ T cells of the AT SVF of lean (n = 10 to 12), overweight (OW; n = 6 to 7), and obese (Ob; n = 12 to 14) patients. Results are means ± SEM. *P < 0.05. E: Intracellular cytokine analysis for IL-17 on immunoselected CD3⁺ T lymphocytes of the AT SVF of OW (n = 6 to 7) and Ob (n = 12 to 14) patients. F: Allogeneic CD4⁺ T cells purified from blood of healthy donors (n = 3) were cultured for 6 days with sorted CD1c⁺ DCs from the CD19⁻CD14⁻ cells of the SVF of obese patients (n = 3). CD4⁺ T cells were collected, stimulated, and stained for intracellular IL-17 and IFN-γ cytokines. The experiment was repeated three times with similar results. SSC-A, side scatter-aire; AU, arbitrary unit.