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. 2012 Aug 17;61(9):2330–2339. doi: 10.2337/db11-1523

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 6. — The influence of obesity on maturation of DC in AT and liver. Isolated mononuclear cells from liver and SVC from AT were stained for markers to define subsets of CD11c⁺ as well as CD86⁺ cells and analyzed by flow cytometry (n = minimum of 6 animals per group). Results are presented as means ± SE, and significant differences are indicated (*P < 0.05). A: Number of CD11c⁺CD86⁺ cells isolated from AT. B: MFI for CD86 on CD11c⁺CD86⁺ cells from AT. C: Number of CD11c⁺CD86⁺ cells isolated from liver. D: MFI for CD86 on liver CD11c⁺CD86⁺ cells. E: Representative flow cytometry plots of CD86⁺ cells in AT, corresponding to A and B. Gate 1a represents CD11c⁺CD86⁺ cells; gate 4a represents CD11b⁺CD11c⁺CD86⁺ cells; and gate 5a represents CD11b⁻CD11c⁺CD86⁺ cells. F: Representative flow cytometry plots of CD86⁺ cells in liver, corresponding to C and D. Gate 1a represents CD11c⁺CD86⁺ cells; gate 4a represents CD11b⁺CD11c⁺CD86⁺ cells; and gate 5a represents CD11b⁻CD11c⁺CD86⁺ cells. FACS, fluorescence-activated cell sorter; APC, allophycocyanin.

FIG. 6. — The influence of obesity on maturation of DC in AT and liver. Isolated mononuclear cells from liver and SVC from AT were stained for markers to define subsets of CD11c⁺ as well as CD86⁺ cells and analyzed by flow cytometry (n = minimum of 6 animals per group). Results are presented as means ± SE, and significant differences are indicated (*P < 0.05). A: Number of CD11c⁺CD86⁺ cells isolated from AT. B: MFI for CD86 on CD11c⁺CD86⁺ cells from AT. C: Number of CD11c⁺CD86⁺ cells isolated from liver. D: MFI for CD86 on liver CD11c⁺CD86⁺ cells. E: Representative flow cytometry plots of CD86⁺ cells in AT, corresponding to A and B. Gate 1a represents CD11c⁺CD86⁺ cells; gate 4a represents CD11b⁺CD11c⁺CD86⁺ cells; and gate 5a represents CD11b⁻CD11c⁺CD86⁺ cells. F: Representative flow cytometry plots of CD86⁺ cells in liver, corresponding to C and D. Gate 1a represents CD11c⁺CD86⁺ cells; gate 4a represents CD11b⁺CD11c⁺CD86⁺ cells; and gate 5a represents CD11b⁻CD11c⁺CD86⁺ cells. FACS, fluorescence-activated cell sorter; APC, allophycocyanin.