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. 2012 Aug 22;7(8):e43613. doi: 10.1371/journal.pone.0043613

Figure 6. Brucella-infected DCs induce Vγ9Vδ2 T cells to produce IFN-γ even when they are added 24 h p.i.

Figure 6

Brucella-infected DCs (MOI = 20) were cultured alone or in the presence of untreated or stimulated (0.2 nM HMB-PP) Vγ9Vδ2 T cells. Vγ9Vδ2 T cells were added to DCs 1 h or 24 h p.i. At 24 h, 48 h and 72 h p.i., supernatants were collected. IFN-γ was assessed by ELISA test. Data shown are the mean +/− SD of triplicates and representative of three independent experiments performed with cells from different donor. A significant difference between infected DCs in the presence or not of Vγ9Vδ2 T cells was calculated by using Student’s t test and is indicated by (**) where p<0.01 and (***) where p<0.001.