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Target cells are prepared on Day 1 by CD8 depleting PBMC and stimulating cells with ConA. Cells are washed on Day 2. On Day 4 target cells are superinfected in vitro with SIVmac239 and plated in a 96 well plate. Effector cells are prepared by isolating CD8β T cells from PBMC. Effector cells are added to target cells in a 1∶1 ratio. Each combination of effector and target cells was compared in this assay.
