Skip to main content
. 2012 Aug 22;7(8):e43645. doi: 10.1371/journal.pone.0043645

Figure 2. The effects of MPT0E028 on cell growth and cell cycle progression in human HCT116 cells.

Figure 2

(A) Concentration-dependent effect of MPT0E028 and SAHA on cell growth. HCT116 cells were incubated without or with the indicated concentrations of MPT0E028 or SAHA for 48 h. Cell growth was evaluated by SRB assay. Data were expressed as mean±S.E.M. of at least 3 independent experiments. (B) Concentration-dependent effects of MPT0E028 and SAHA on cell cycle progression. HCT116 cells were treated without or with the indicated concentrations of MPT0E028 or SAHA for 24 h and were analyzed by flow cytometry for cell cycle distribution. (C, D) Data shown are the means of at least 3 independent experiments. (E) Time-dependent effects of MPT0E028 and SAHA on subG1 population. HCT116 cells were treated without or with 1 µM MPT0E028 or SAHA for the indicated time interval and were analyzed by flow cytometry for subG1 population. (F) MPT0E028 induced-caspase 3 and PARP activation. HCT116 cells were treated without or with the indicated concentration of MPT0E028 or SAHA for 24 h subject to western blot for caspase 3 and PARP analysis. (G) MPT0E028 induced casepase-dependent cell apoptosis. HCT116 cells were treated without or with 3 µM MPTE028, SAHA or 20 µM z-VAD-fmk for 24 h and subjected to western blot for caspase 3 and PARP analysis.