Table 2. Activity of MPT0E028 and SAHA against HDACs.

Compound	HDAC1	HDAC2	HDAC8	HDAC4	HDAC6
	Class I			Class IIa	Class IIb
	IC50 (nM±SE)
MPT0E028	53.0±12.0	106.2±23.0	2532.6±314.7	>10000	29.5±6.4
SAHA	102.2±22.1	336.2±91.2	2898.9±128.7	>10000	19.5±3.1

HDAC activity was determined indirectly by measuring the fluorescence generated by a deacetylated fluorogenic peptide product (Materials and Methods). Data represent triplicate determinations from three independent experiments (mean±SE). HDAC Class I isoforms: HDAC1, HDAC2, and HDAC8, HDAC Class II isoforms: HDAC4 and HDAC6.