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. 2012 Aug 22;7(8):e43374. doi: 10.1371/journal.pone.0043374

Figure 1. Effect of uremic media on the activation of p38MAPK signaling pathway.

Figure 1

A. Ratios of phosphorylated p38 MAPK relative to the total protein, obtained in EC after 1 and 15 min of incubation with pooled sera from control donors (C), and predialysis (PreD), hemodialysis (HD), and peritoneal dialysis (PD) groups of patients. Data are corrected for cell number and are represented as mean±SEM (n = 6); *p<0.05 when compared with the other groups. B. EC were incubated at different time points with pooled sera from the same groups (C, PreD, HD, and PD, as indicated), and then assayed for phospho-p38 MAPK in cytosolic fractions by Western blot analysis. Images are representative of 6 different experiments and bar diagrams represent the mean intensity of phosphorylation (*p<0.05, **p<0.001).