Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Detection of EFEMP2 and PITX2 expression in ocular cell lines by RT–PCR. Intron-flanking primers were used to amplify mRNA transcripts of EFEMP2 (161 bp) and PITX2 (133 bp) from various transformed cell lines, using the housekeeping gene HPRT1 (130 bp) as a control. Reverse-transcribed (RT) samples either contained (+) or lacked (-) enzyme, and control templates of genomic DNA and water were included, to verify that amplified products were a result of specific primer binding.
