Table 2. Overview mosquito feeding procedures.
| Country | Timing of membrane feeding | Mosquito species | Mosquito origin | Membrane | Mercuro-chrome | Mosquitoes dissected per experiment, median (IQR) | Number of feeds (infected) | ||||||
| skin | whole | own | control | skin | whole | own | control | ||||||
| Cameroon-1 [30] | Enrolment | An gambiae s.s. M form | Colony | Parafilm® | 2% | ND | 30(28–30) | 30(23–30) | 29(21–30) | ND | 140 (75) | 110 (47) | 106 (61) |
| Cameroon-2 [23] | Enrolment | An gambiae s.s. M form | Colony | Parafilm® | 3% | 27(18–30) | 29(17–30) | ND | ND | 47 (27) | 34 (24) | ND | ND |
| Cameroon-3 [29] | Enrolment | An gambiae s.s. M form | Colony | Parafilm® | 2% | ND | 30(29–30) | 29(26–30) | 29(24–30) | ND | 58 (50) | 58 (39) | 58 (57) |
| Cameroon-4 [33] | Enrolment | An gambiae s.s. M form | Colony | Parafilm® | 2% | 30(27–30) | 30(26–30) | 29(24–30) | 29(23–30) | 55 (55) | 39 (39) | 30 (30) | 31 (31) |
| Cameroon-5 (Gouagna et al., unpublished) | Enrolment | An gambiae s.s., M form | Colony | Parafilm® | 2% | 21(15–25) | 20(20–25) | ND | ND | 24 (17) | 22 (13) | ND | ND |
| Cameroon-6 (Morlais et al., unpublished) | Enrolment | An gambiae s.s. M form | Colony | Parafilm® | 0.4% | ND | 57(43–70) | 54(43–60) | 56(47–64) | ND | 25 (22) | 24 (22) | 25 (25) |
| Mali [25], [34] | Enrolment | An gambiae s.l. | F1 progeny | Parafilm® | 0.5% | 78(51–92) | 75(58–116) | ND | ND | 70 (59) | 70 (54) | ND | ND |
| Papua New Guinea [26] | Enrolment | An farauti | Colony | Baudruche® | 0.2% | NA | NA | ND | ND | NA | NA | ND | ND |
| Senegal [27] | Enrolment | An. arabiensis | Larval collections | Baudruche® | 1% | 14(10–24) | 6(3–10) | ND | ND | 60 (25) | 56 (17) | ND | ND |
| The Gambia [17], [28], [38], [39] | After treatment | An. gambiae s.l. | F1 progeny | Parafilm® | 0% or 2% | ND | ND | 18(13–23) | 19(13–23) | ND | ND | 348 (95) | 366 (129) |
IQR = interquartile range (25th and 75th percentile). Skin = direct skin feeding; whole = membrane feeding with whole blood sample; own = membrane feeding with serum replacement, red blood cells are re-suspended in the donor's own (autologous) plasma; control = membrane feeding with serum replacement, red blood cells are re-suspended in malaria-naïve control serum; infected = at least one infected mosquito; NA = not available; ND = not done.