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. 2012 Aug 22;7(8):e43155. doi: 10.1371/journal.pone.0043155

Figure 2. Comparison of hLIF, hOSM, mOSM and rOSM activated signaling pathways in primary dermal fibroblasts.

Figure 2

A, Rat dermal fibroblasts (RDF) and B, human dermal fibroblasts (HDF) were treated with 10 ng/ml hLIF, hOSM, mOSM or rOSM for 15 min. The phosphorylation levels of STAT1, STAT3, ERK1/2 as well as STAT5, p38 and Akt were detected via Western blot analysis. The blots were stripped and reprobed with antibodies recognizing the proteins irrespective of their activation status. Additionally an α-tubulin loading control was included. Phosphorylation intensities were quantified by chemiluminescence analysis and normalized to tubulin. Activation determined for rOSM was set to 100%. Shown are the means (n = 3) with standard error of mean (SEM). * p<0.05, ** p<0.01, *** p<0.001 untreated vs. cytokine-treated sample.