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. 2012 Aug 22;7(8):e43417. doi: 10.1371/journal.pone.0043417

Figure 3. GABAergic signaling at the Golgi cell – granule cell synapse is still transiently inhibited following high frequency stimulation when mGluRs are blocked. A,

Figure 3

GABAergic IPSCs were evoked in granule cells by stimulating Golgi cell axons at 0.1 Hz. After including LY341495 (100 µM) in the cocktail of antagonists (see Methods), high frequency stimulation (HFS, 10 pulses at 100 Hz) still reduced both eIPSCs and Itonic current. Depicted responses are averages of 5 to 10 eIPSCs, before (c1, c2) and after HFS, at 5 min (t1), 20 min (t2), and 40 min (t3). B , Histograms show averaged data from 4 cells for eIPSCs (black) and 4 cells for Itonic (red). C, Time course of the changes in eIPSC amplitude following HFS for a representative cell, and D, Histograms showing averaged data from 4 cells for eIPSCs compared to data from the same experiment without LY341495 (Fig. 2). E, Time course of the changes in Itonic for a single cell, and F, averaged values for 4 cells in comparison to the same experiment without LY341495.