Fig. 1.

A representative Western blot of the intracellular expression of Prdx2 in blood lymphocyte lysates from RA patients compared with healthy subjects. Lysates of lymphocytes from RA patients and healthy subjects (HS) were loaded (30 μg total protein/lane) onto 4–20% gradient SDS-PAGE gels under non-reducing conditions and analyzed by Western blotting with anti-Prdx2. Molecular mass markers are denoted by ‘M’. Samples of purified human Prdx2 (10, 25 and 100 ng/lane) were loaded as standards on each blot. Equal loading of total protein was confirmed by the Bradford assay and aliquots of the lysates were resolved on SDS-PAGE under reducing conditions, blotted and probed with anti-tubulin. The bands in this tubulin blot image originate from two blots and have been moved around to match the order of samples in the Prdx2 blot.