Skip to main content
. 2012 Aug;44(8):1223–1231. doi: 10.1016/j.biocel.2012.04.016

Fig. 3.

Fig. 3

Presence of exofacial Prdx2 and Trx1 on lymphocytes. (A) Representative flow cytometry dot plots of the presence of exofacial Prdx2 and Trx1 on T and B lymphocytes. The Y-axes show the proportion of lymphocytes with a T cell (CD3-PE) or B cell (CD19-PE) phenotype. The X-axes show the proportion of lymphocytes that stain for cell surface Trx1 (Trx1-FITC indirect staining) or Prdx2 (Prdx2-FITC indirect staining). Thus the upper right-hand quadrant of each graph shows the proportion of T or B cells that stain for Trx1 or Prdx2. The numbers in each graph represent the percentage of cells contained in each quadrant. (B) The % of T and B lymphocytes in RA and healthy (HS) lymphocytes with exofacial Prdx2 is shown. Horizontal lines indicate median values. Two asterisks indicate a statistically significant difference at the p < 0.01 level (Mann–Whitney U-test). (C) The % of T and B lymphocytes in RA and healthy (HS) lymphocytes with exofacial Trx1 is shown. Horizontal lines indicate median values. Two asterisks indicate a statistically significant difference at the p < 0.01 level (Mann–Whitney U-test). (D) Immunohistochemical detection of exofacial Prdx2 and Trx1, and intracellular Prdx2 in peripheral blood lymphocytes from a healthy subject. PBLs were isolated from peripheral blood. Exofacial Prdx2 (panel e) and Trx1 (panel h) was detected on live cells using mouse monoclonal antibodies to human Prdx2 and Trx1, respectively, in conjunction with a FITC-conjugated goat anti-mouse Fab2 fragment of IgG. Cells labeled with the secondary antibody only were used as controls (panels a–c). After labeling, the cells were fixed with 4% paraformaldehyde and mounted on slides with antifade fluid supplemented with DAPI for nuclear staining (panels a, d, g). Cells permeabilized and fixed with methanol were used to label intracellular Prdx2 (panel k). The permeabilized and labeled cells were mounted on slides with antifade fluid supplemented with DAPI for nuclear staining (panel j). The arrows in panels f, i, and l depict DAPI-stained cells that were positive for exofacial Prdx2 (panel f), exofacial Trx1 (panel i), or intracellular Prdx2 (panel l), respectively.