FIGURE 1.

The methylation status of guanosine cap nucleotides in Saccharomyces cerevisiae (yeast) and two strains of Trichomonas vaginalis (T1 and G3). In vivo-labeled RNAs were immunoprecipitated by anti-TMG. The guanylate cap was released by TAP and analyzed by two-dimensional thin-layer chromatography (2D-TLC) with solvents [A] and [B], as described in the Materials and Methods. The migration of the four unmodified rNMPs (A, C, G, and U), as detected by UV shading, is plotted on every image for reference (see Materials and Methods). The far right panel (standards, only) denotes the three methylation status of the guanosine cap (M: m⁷GMP; D: m^2,7GMP, and T: m^2,2,7GMP) relative to the migration of A, C, G, and U as described in the Materials and Methods.