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. 2012 Sep;18(9):1702–1715. doi: 10.1261/rna.034314.112

FIGURE 3.

FIGURE 3.

The Rck/p54 QN-rich extension is dispensable for P-body assembly. (A) Localization in pre-existing P-bodies in the absence of the QN-rich region. HeLa cells were transfected with expression vectors encoding RFP-tagged human wild-type (p54) and truncated (p54-ΔQN) Rck/p54, and Drosophila Me31B. After 40 h, P-bodies were stained with anti-Ge1 antibodies and observed by fluorescence microscopy. Bar, 20 μm. (B,C) De novo assembly of P-bodies in the absence of the QN-rich region. HeLa cells were transfected with si-p54 to deplete Rck/p54 and suppress P-bodies. Twenty-four hours later, cells were transfected as described in A and stained with anti-Ge1 antibodies. (C) P-bodies assembled with Me31B do not contain human Rck/p54. P-bodies were assembled with Me31B as described in B and stained with anti-p54 antibodies.