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. 2012 Sep;18(9):1716–1724. doi: 10.1261/rna.034132.112

FIGURE 1.

FIGURE 1.

Alignment of putative ACNase subunits of killer toxins from Pichia acaciae, Debaryomyces robertsiae, Kluyveromyces lactis, and Pichia inositovora. For the multiple alignment, ClustalV (PAM250) was applied. Positions which have the same amino acid in at least two of the four proteins are shaded gray. Positions of side chain identity in all four proteins are shown in boxes. Negative positions represent the predicted signal peptide of each protein; position +1 accounts for the methionine added to achieve intracellular expression of variants lacking the signal peptide. Numbering of conserved residues refers to the intracellular version of PaOrf2-(1-319). NCBI GenBank accession numbers: P. acaciae pPac1-2 ORF2, CAE84960.1; D. robertsiae pWR1A ORF3, CAE84956.1; K. lactis pGKL1 ORF4, YP_001648056.1; P. inositovora pPin1-3 ORF4, CAD91887.1.