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. 2012 Jul 18;2012:538129. doi: 10.1155/2012/538129

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Copyright © 2012 Atsushi Ogawa et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Schematic sequence of the tRNA-fused template with two T7 promoters. The triple N in red represents the anticodon- or anticodon-loop-randomized region. (b) Agarose gel electrophoretic assay of the template DNAs recovered from tRNA_CUA-fused template (lane 1), nonfused template lacking the tRNA domain (lane 2), a 1 : 1 mixture thereof (lanes 4, 5), or tRNA_CGA-fused template (lane 3), after coupled transcription/translation under normal (noncompartmentalized) (lanes 1–4) or compartmentalized (lane 5) conditions.