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. 2012 Jul 18;2012:538129. doi: 10.1155/2012/538129

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Copyright © 2012 Atsushi Ogawa et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Summary of selection/amplification of anticodon-randomized or anticodon-matched/anticodon-loop-randomized tRNAs for Ser. The numbers of clones that possess the sequence shown are indicated in parentheses. (b) Western blotting analysis of the translation of nonfused (tRNA-lacking) mRNA templates under normal (noncompartmentalized) conditions with (lanes 9–15) or without (lane 8) tRNA; tRNA^SerU _CUA (lane9), tRNA^SerU _CGA (natural tRNA for Ser, lane 10), anticodon-loop-randomized tRNA^SerU after 0, 3, or 5 rounds of selection/amplification (lanes 11, 12, or 13, respectively), or anticodon-matched but singly loop-mutated tRNA^SerU (lanes 14 and 15). Lane 7 represents a control translation using amber-free mRNA with no use of tRNA as a positive control and lanes 1–7 are a calibration set.