Table 2.
Histomorphometric analysis for B6 and BALB P2X7+/+ and P2X7−/− mice.
| B6 | B6+/+ | B6−/− | t-Test | ||
|---|---|---|---|---|---|
| Vertebrae | Mean | SEM | Mean | SEM | P |
|
| |||||
| N | 12 | 8 | |||
| MS/BS% | 43.8 | 2.9 | 42.5 | 3.9 | 0.796 |
| MAR (μm/day) | 1.07 | 0.03 | 1.00 | 0.04 | 0.077 |
| ES/BS (%) | 7.9 | 0.4 | 9.1 | 0.7 | 0.180 |
| BV/TV (%) | 17.7 | 1.5 | 20.4 | 0.9 | 0.122 |
| Tb.Th. (μm) | 32.8 | 1.1 | 36.5 | 0.7 | 0.011 |
| C.Th. (μm) | 93.0 | 3.6 | 93.7 | 4.8 | 0.917 |
|
| |||||
| Tibia | Mean | SEM | Mean | SEM | P |
|
| |||||
| N | 12 | 8 | |||
| MS/BS% | 46.1 | 2.1 | 44 | 2.4 | 0.517 |
| MAR (μm/day) | 1.16 | 0.03 | 1.2 | 0.04 | 0.378 |
| ES/BS (%) | 8.3 | 0.6 | 8.0 | 0.3 | 0.676 |
| BV/TV (%) | 10.0 | 1.7 | 13.2 | 1.7 | 0.197 |
| Tb.Th. (μm) | 32.3 | 1.1 | 35.5 | 1.7 | 0.138 |
| C.Th. (μm) | 127.4 | 3.8 | 162.6 | 6.1 | <0.001 |
|
| |||||
| BALB/cJ | BALB/cJ+/+ | BALB/cJ−/− | t-Test | ||
|
| |||||
| Tibia | Mean | SEM | Mean | SEM | t-Test |
|
| |||||
| N | 7 | 7 | |||
| MS/BS% | 44.8 | 5.7 | 53.4 | 4.1 | 0.267 |
| MAR (μm/day) | 1.00 | 0.03 | ND | — | |
| ES/BS (%) | 10.15 | 0.5 | 9.9 | 0.9 | 0.791 |
| BV/TV (%) | 8.7 | 1.7 | 9.5 | 1.9 | 0.766 |
| Tb.Th. (μm) | 31.1 | 2.1 | 33.1 | 1.1 | 0.423 |
| C.Th. (μm) | 148.9 | 10.4 | 139.8 | 8.9 | 0.517 |
Changes in histomorphometric parameters in P2X7−/− compared to P2X7+/+ in the tibia and vertebral (only B6). Displayed as mean ± SE for the group. The direction of changes is equal in the different regions of the B6 mice, besides MAR where it is decreased in the vertebral region of P2X7−/− and decreased in the tibia. The ES/BS (%) has the opposite directions in vertebrae and tibia. Besides the cortical thickness and MS/BS (%) the directions of changes are the opposite in the BALB mice compared to the B6 mice. The size of the changes in the histomorphometric parameters P2X7−/− mice is different in relation to region and strain analyzed. Significant differences from wild type animals at the P < 0.05 levels, determined by Student t-test, are displayed.