Figure 3.

Cell cycle arrest and reduced DNA synthetic rate of Blp-deficient S2 cells. A, B) Flow cytometric analysis of cells after Blp- or LASP-RNAi treatment stained with propidium iodide (A), and quantitation of the percentage of cells in different phases of the cell cycle (B), showing significant accumulation in both G1 and S phases of the cell cycle at d 6 and 9 and a reduction of cells in G2/M phases. LASP-RNAi treatment did not affect the cell cycle profile. Values are means ± sd; n = 3. *P < 0.001; Student's t test. C) Cellular BrdU incorporation rate (30 min labeling with BrdU) after Blp-RNAi treatment, showing significantly reduced numbers of BrdU⁺ cells at d 6 and 9, indicating fewer cells undergoing active DNA synthesis during the labeling period, leading to a reduced number of cells incorporating BrdU. Only BrdU⁺ cells were gated for analysis.