Figure 2.

Agonist-induced global cAMP accumulation and effects of AKAP disruption. Multiple HASM lines were infected with retrovirus enabling expression of scramble peptide (SCR) or the AKAP disrupting peptides AKAP-IS or Ht31. Global cAMP accumulation was measured after 10 min stimulation with ISO (50 nM or 1 μM), or FSK (100 μM). Experiments were done in the absence or presence of the broad PDE inhibitor IMBX (1 mM) or the selective PDE4 inhibitor RO 20-1724 (1 mM). Data are presented as mean ± se values, n = 5.