TABLE 1.

Investigation of AKAP isoform expression by real-time PCR

Isoform	Ct	Ct GAPDH	ΔCt	n
AKAP1	26.40 ± 1.10	18.18 ± 1.24	8.22 ± 1.66	10
AKAP2	25.00 ± 1.14	18.18 ± 1.24	6.82 ± 1.68	10
AKAP10	24.09 ± 0.56	17.37 ± 0.84	6.72 ± 1.01	6
AKAP11	25.53 ± 1.14	18.18 ± 1.24	7.35 ± 1.68	10
AKAP12	20.80 ± 1.29	18.18 ± 1.24	2.62 ± 1.79	10
AKAP13	24.34 ± 1.34	18.18 ± 1.24	6.16 ± 1.83	10
Ezrin	23.06 ± 1.44	18.18 ± 1.24	4.88 ± 1.90	10
MAP2B	25.16 ± 1.02	17.37 ± 0.84	7.79 ± 1.32	6

Multiple HASM cultures were analyzed for mRNA levels of the indicated AKAPs. Data are presented as mean ± se values.