TABLE 1.

Mitochondrial respiration rates of lung homogenates

Substrate	State	WT	HT	KO
Pyruvate + malate (10 mM each)	Endogenous	2.28 ± 0.73	1.53 ± 0.47	1.95 ± 0.52
	Substrates	2.43 ± 0.42	1.87 ± 0.58	2.16 ± 0.34
	ADP	10.85 ± 1.51	9.07 ± 2.48	11.23 ± 0.74
	Oligomycin	1.50 ± 0.33	1.28 ± 0.40	1.60 ± 0.18
	RCR	7.62 ± 1.87	7.37 ± 1.48	7.14 ± 1.08
Succinate (10 mM)	Endogenous	2.70 ± 0.74	2.18 ± 0.60	2.34 ± 0.52
	Substrates	6.85 ± 0.94	5.29 ± 1.05*	6.10 ± 0.45
	ADP	24.37 ± 2.81	18.79 ± 3.63*	22.20 ± 2.03
	Oligomycin	3.79 ± 0.75	2.81 ± 0.30*	3.23 ± 0.24#
	RCR	6.57 ± 0.93	6.65 ± 0.74	6.87 ± 0.30
Glycerol 3-phosphate (10 mM)	Endogenous	2.44 ± 0.65	2.14 ± 0.28	2.24 ± 0.32
	Substrates	2.81 ± 0.54	2.45 ± 0.55	2.65 ± 0.59
	ADP	8.35 ± 0.92	6.93 ± 1.56	7.41 ± 1.14
	Oligomycin	2.45 ± 0.57	2.13 ± 0.56	2.14 ± 0.48
	RCR	3.53 ± 0.56	3.29 ± 0.20	3.53 ± 0.34

Data are presented as means ± se (n=4). WT, wild type; HT, heterozygote; KO, knockout. Respiration rate is defined as picomoles oxygen consumed per second per milligram wet tissue. Measurements were performed with a final concentration of 3.5 mg protein/ml.

^*P ≤ 0.05 vs. WT;

^#P ≤ 0.05 vs. HT.