Figure 2.

Mouse ovarian expression of PLA₂G4A and PTGS2 and regulation by gonadotropins. Ovarian expression of PLA₂G4A and PTGS2 proteins and their regulation by gonadotropins were determined by Western blot analysis. A) PLA₂G4A expression by the Pla2g4a^+/+ ovary in unstimulated and stimulated conditions, with Pla2g4a^−/− ovary as negative control. M, PLA₂G4A standard protein. B) Pla2g4a^+/+ ovarian expression of PLA₂G4A, quantified as fold induction relative to that in unstimulated ovaries (means±se, n=3). *P < 0.05 vs. pre-eCG group. C) PTGS2 expression by the mouse ovary during the time course of gonadotropin treatment. D) Comparison in PTGS2 level at hCG6h among genotypes was made from three different samples (relative to that of Pla2g4a^−/− ovary, means±se, n=3). *P < 0.05 vs. Pla2g4a^−/− group at hCG6h.