Figure 4.

Inhibition by AACOCF₃ or indomethacin of PTGS2 induction and ovulation in rats. Effects of local AACOCF₃ or systemic indomethacin on ovulation rate (A) and PTGS2 induction (B–F) were investigated in superovulated rats. See Materials and Methods for details. A) Both AACOCF₃ (AACO) and indomethacin (indo) significantly reduced ovulation rate. In experiments with local treatment, data were transformed as percentage of oocyte numbers of each contralateral (cont) intact ovary (n=6). In experiments with systemic treatment, data were transformed as percentage of the mean of vehicle (veh)-treated rats (n=6 in vehicle-treated group, n=4 in indomethacin-treated group). Data are shown as means ± se. NS, not significant. **P < 0.01 vs. intact control; paired t test. B–F) PTGS2 immunoreactivity in intact animals at hCG0h (B) and hCG8h (C) and in vehicle (local; D)-, AACOCF₃ (E)-, or indomethacin (F)-treated follicles at hCG8h. Each photo was taken with the same camera with identical lighting and exposure time. Semiquantitative comparison in staining intensity among treatment groups is summarized in Table 2. Scale bar = 200 μm.