Figure 6.

Semiquantitative analysis of expression of immunoreactive PTGS2 and 3 PLA₂ isoforms in rat preovulatory follicles. Serial sections of rat ovaries at hCG8h were immunostained for PTGS2, PLA₂G1B, PLA₂G2A, and PLA₂G4A. A total of 47 antral and graafian follicles in sections of 3 different ovarian samples (for example, Fig. 5B) were numbered, and the intensities of immunoreaction for each enzyme in each follicle were evaluated on a scale of 4 grades (negative, slightly positive, moderately positive, intensely positive) with different blue colors. One investigator scored the sections 2 or 3 times, and another investigator scored them independently. The readings resulted in a high level of agreement.