TABLE 2.
PTGS-2 immunoreactivity in rat preovulatory follicles
| Time | Group | Ovaries | Score |
P | |||
|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | ||||
| hCG0h | Untreated | 3 | 20 | 0 | 0 | 0 | |
| hCG8h | Vehicle | 4 | 5 | 3 | 5 | 19 | |
| hCG8h | AACOCF3 | 4 | 2 | 8 | 7 | 5 | <0.05 |
| hCG8h | Indomethacin | 5 | 6 | 4 | 3 | 7 | >0.05, <0.07 |
Preovulatory follicles that looked healthy and had an axis > 700 μm were examined (numbers of ovaries in each group are indicated). Staining intensity, as shown in Fig. 4, was evaluated semiquantitatively; scores, with a scale graded from 0 (negative), 1 (faintly positive), 2 (moderately positive) to 3 (strongly positive), were statistically compared between vehicle- and inhibitor (AACOCF3 or indomethacin)-treated groups by Mann-Whitney U test.