Skip to main content
. 2012 Sep;26(9):3875–3883. doi: 10.1096/fj.12-205658

Figure 2.

Figure 2.

TLC analysis of soluble nucleotidase activities in CD39−/− mice. A, B) Sera from CD39−/− and wild-type (CD39+/+) mice were incubated with 50 μM [γ-32P]ATP (A) or with 100 μM [3H]ADP (B). Some samples were pretreated with EDTA (2 mM), POM-1 (100 μM), or Ap5A (80 μM) prior to addition of tracer substrates. Blanks (Bl) show the radiochemical purities of [γ-32P]ATP and [3H]ADP. Arrows indicate the positions of nucleotide and nucleoside standards, inorganic phosphate (Pi) and pyrophosphate (PPi). C) Rate of [3H]ADP (100 μM) hydrolysis was measured in sera from CD39+/+ and CD39−/− mice in the absence (left panel) or presence (middle panel) of 80 μM Ap5A, while 5′-nucleotidase (5-NT) was assayed using 300 μM [3H]AMP as substrate (right panel). Data are presented as means ± se (n=12). *P < 0.05 vs. wild-type controls.