Figure 5.

Downregulation of CDT1 Genes Results in Endogenous DNA Stress.

(A) Expression of CycB1;1 was monitored by quantitative RT-PCR. Total RNA was extracted from plantlets. Data presented here are averages of two technical replicates obtained from two biological replicates (black and gray bars). Expression of CycB1;1 was reproducibly found to be increased in CDT1-RNAi and cdt1a/+cdt1b mutants. The increase observed in the second experiment in cdt1a/+ mutants was not reproducible, suggesting that expression of CycB1;1 varies a little in this background.

(B) Expression of genes involved in various DNA repair pathways was monitored by quantitative RT-PCR. All genes were found to be induced in CDT1-RNAi lines (black bars) compared with the wild type (Wassilewskija [Ws]; gray bars). Data presented here represent two biological replicate experiments. Each quantitative RT-PCR was repeated at least twice on each biological replicate.

For all panels, bars are averages ± sd.