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Altered Isoelectric Focusing of DOG1 Protein during After-Ripening.
Freshly harvested or 12 weeks after-ripened seeds from transgenic plants containing the ProDOG1:3xHA:DOG1 construct were imbibed for 16 h. Extracted proteins were analyzed by 2D gel electrophoresis/immunoblotting using anti-HA antibody. pH values and molecular mass markers are indicated above and on the left of the blots, respectively. AR, after-ripened.
