Figure 2.

Bipartite NLS and NES Are Involved in the Sugar-Regulated Cellular Targeting of MYBS1.

Barley aleurones were transfected with Ubi:GFP-OsMYBS1 (full length, truncated, or mutated) and incubated in +sugar or −sugar medium for 24 h. Amino acids highlighted in red are conserved residues for NLS or NES, and those underlined in green are substituting residues. a, GFP with a punctate pattern; C, cytoplasm; C/N, nucleus and cytoplasm; N, nucleus; V, vacuole.

(A) Nucleocytoplasmic partitioning of different forms of MYBS1 fused to GFP.

(B) MYBS1(108-212) contains a bipartite NLS.

(C) MYBS1 contains a bipartite NES.