Figure 2.

Role of Cytokinin in Ovule Development.

(A) Wild-type ovule at stage 3-V. The dotted line indicates the female gametophyte.

(B) cre1-12 ahk2-2 ahk3-3 ovule at stage 3-V. The female gametophyte arrested at stage FG1 (arrowhead).

(C) cre1-12 ahk2-2 ahk3-3 finger-like structure.

(D) pin1-5 ovule at stage 3-V. The female gametophyte arrested at stage FG1 (arrowhead).

(E) pin1-5 finger-like structure.

(F) Wild-type ovule expressing PIN1pro:PIN1-GFP.

(G) cre1-12/+ ahk2-2/+ ahk3-3/+ ovule expressing PIN1pro:PIN1-GFP.

(H) cre1-12 ahk2-2 ahk3-3 triple mutant ovule expressing PIN1pro:PIN1-GFP.

(I) spl ovule expressing PIN1pro:PIN1-GFP.

(F) to (I) Pictures taken using the bright field (left) and the dark field (right). The dotted line shows the ovule profile.

chal, chalaza; f, funiculus; fg, female gametophyte; ii, inner integument; nu, nucellus; oi, outer integument.

Bars = 20 μm.