Figure 2.

Immunoblots Demonstrating the Relative Amounts of Chloroplast Proteins between the Wild Type and the pgr5 Mutant.

(A) Immunoblots of the PsaB and D1 proteins from mature leaves of the wild type and pgr5. Plants were grown under constant low light (50 µmol photons m⁻² s⁻¹) (LL), moderate growth light (120 µmol photons m⁻² s⁻¹) (GL), high light (500 µmol photons m⁻² s⁻¹) (HL), or mildly fluctuating light (mFL) with 5 min of low (50 µmol photons m⁻² s⁻¹) and 1 min of moderate high (350 µmol photons m⁻² s⁻¹) light repeating for the entire photoperiod. Col-0, Columbia-0.

(B) and (C) The amounts of the chloroplast proteins representing the major photosynthetic protein complexes (B) and alternative electron transfer routes (C) in the wild type and pgr5. The seedlings were grown for 9 d under constant (200 µmol photons m⁻² s⁻¹) or under fluctuating light with 5 min of low light (50 µmol photons m⁻² s⁻¹) and 1 min of high light (500 µmol photons m⁻² s⁻¹) repeating during the entire photoperiod. Gels were loaded on equal protein basis for the wild type (100%) and pgr5. For each protein, a representative example of at least three biological replications is shown, including the dilution series (50 to 200%) made with the wild-type Col-0 leaf extracts.