Figure 5.
Degradation of phyA/phyB Chimeric Proteins under Continuous R.
(A) Immunoblot detection of the phyA/phyB chimeric proteins in seedlings treated with continuous R. Three-day-old, dark-grown seedlings were kept in the dark (D) or exposed to R (8.5 μmol/m2/s) for 24 h. The blots were probed with anti-GFP monoclonal antibodies (top). Five micrograms of total protein was loaded in each lane. To confirm equal protein loading, the same blots were subjected to Coomassie blue (CBB) staining (bottom).
(B) Expression levels were estimated by densitometric analysis of the immunoblots using a dilution series. The data are presented relative to the dark levels. Data are the means ± se (n = 3).