Figure 5.
Expression of Genes in the cry1 Regulon in the cry1, zml2-1, zml2-2, zml1, and zim Mutant Lines after Exposure to High Light.
Wild-type and the different mutant seedlings were grown for 10 d in continuous white light (100 μmol quanta m−2 s−1) at 23°C and shifted to excess light (3 h 1000 μmol quanta m−2 s−1). Expression of genes from the cry1-dependent regulon ELIP2 (At4g14690) (A), GPX7 (At4g31870) (B), ERD9 (At1g10370) (C), and MYB7 (At1g56650) (D) as well as the cry1-independent regulon RBCS (At1g67090) (E) and LHCB2.4 (At3g27690) (F) were analyzed using real-time PCR. The expression following exposure to high light was related to the control of each genotype. The results were normalized to the expression level of At4g36800 encoding a ubiquitin-protein ligase-like protein. The mean (±sd) of at least three biological replicates is shown. WT, the wild type.