Figure 6.

MPPR6 Expression in the appr6-3 Mutant Background.

(A) Diagram of the 35S:mppr6 transgene and appr6-3 mutant locus. The positions of gene-specific and T-DNA primers used for genotyping and RT-PCR are shown by arrows. The T-DNA insertion is indicated by a triangle.

(B) Representative genomic and RT-PCR analysis of 35S:mppr6 harboring appr6-3/+ and appr6-3/− plants and a Columbia-0 plant. Genomic PCR with MPPR6-specific primers confirms the success of transformation. A PCR reaction with three primers was used to distinguish between wild-type, appr6/+, and appr6/− plants. mppr6 and appr6 transcripts were detected by RT-PCR in leaf tissue. Genomic DNA (gDNA) contaminations in the RNA used for RT-PCR were excluded by a control PCR with RNA as template.

(C) Phenotype of 3-week-old wild-type (left) and 3-week-old appr6-3/− plants (right) genetically complemented by MPPR6 expression.