Figure 7.

Expression Level of MPPR6 in Several Maize Tissues Determined by RT-PCR.

Total RNA from diverse maize tissues was used in reverse transcription reactions. First-strand cDNA was applied to a low cycle RT-PCR using MPPR6-specific primers. The resulting products were electrophoretically separated, blotted to a nylon membrane, and hybridized with a DIG-labeled MPPR6-specific probe (probe is diagrammed in Figure 4A). The quality check of the cDNA by a control RT-PCR using primers specific to the housekeeping maize gene jr27 (Lorbiecke et al., 2005) is shown below. RNA quality is indicated below by staining of the rRNA with ethidium bromide. Proportional decreasing of the signal to the imputed RNA dilutions of two samples (4 and 8 DAP) is shown in the bottommost panel.