Figure 2.

Structure and Catalytic Efficiency of the Engineered MOMTs.

(A) The tertiary structure of the fashioned MOMT3 mutant variant. MOMT3 forms a dimer with an interface contributed by the small N-terminal domains from dyad molecules. Two chains are presented in green and cyan. The phenolic substrate (coniferyl alcohol) bound in the active site is depicted in magenta, and the methyl donor product SAH is color coded as C in yellow, N in blue, and O in red.

(B) A close-up view of the active site of MOMT3 in complex with the substrate coniferyl alcohol and the product of the methyl donor SAH. The three amino acid substitutions are labeled in red. The image is acquired from chain B of the dimer.

(C) The catalytic efficiency (k_cat/K_m) of the created MOMT variants in methylating coniferyl alcohol and sinapyl alcohol. For the detailed kinetic parameters, see Supplemental Table 1 online.