Figure 6.

Accumulation of Wall-Bound Phenolics in MOMT Overexpression Plants.

(A) to (D) HPLC profiles of wall-bound phenolics from the cell wall residues of the control (A) and MOMT4 transgenic plants at the absorbance of A₃₃₀ (B). P1 is tentatively identified as 4-O-methyl-ferulic acid, and P2 is tentatively 4-O-methyl-sinapic acid. The mass spectra of P1 and P2 are shown in (C) and (D). The UV spectra are shown in Supplemental Figure 7 online. F, ferulic acid; st, internal standard; trans-C, trans-p-coumaric acid. mAu, milli-absorbance unit.

(E) The quantification of novel 4-O-methyl wall-bound phenolic esters released from the cell wall of MOMT transgenic stems. The data are the means and se of three or four replicates. Me-F, 4-O-methyl-ferulate; Me-S, 4-O-methyl-sinapate. CWR, cell wall residue.

[See online article for color version of this figure.]