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Activity measurement of the acetyl-CoA synthetase via auxiliary enzymes. The decrease of the absorption of NADH was tracked photometrically at 340 nm.
| Enzyme | Reaction catalyzed |
|---|---|
| Acetyl-CoA synthetase | acetate + ATP + CoA ⇌ acetyl-CoA + PPi+ AMP |
| Myokinase | AMP + ATP ⇌ 2 ADP |
| Pyruvate kinase | ADP + PEP ⇌ pyruvate + ATP |
| Lactate dehydrogenase | pyruvate + NADH ⇌ lactate + NAD+ |
