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. 2012 Aug;22(4):275–282. doi: 10.1089/nat.2012.0355

FIG. 1.

FIG. 1.

Live human breast adenocarcinoma (MCF7) cells treated with the AF546-labeled aptamer or 5′-biotinylated aptamer plus streptavidin-AF546. (A) merged fluorescence and differential interference contrast (DIC) microscopy; (B) shows only fluorescence microscopy of AF546-labeled aptamer cell membrane decoration within 5 minutes of aptamer addition. (C) and (D) live MCF7 cells treated with biotinylated aptamer followed by AF546-labeled streptavidin and demonstrating aptamer-dependent labeling of MCF7 cell membranes (C merged fluorescence and DIC; D, fluorescence only). Each image is a z-stack projection of ten 500-nm-thick optical sections.