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Immunoblot analysis of hybridoma supernatants. Screening of anti-major histocompatibility complex class I chain-related gene A (MICA)-producing hybridomas by immunoblot was performed by pool. Each lane represents the reaction of a pool of 12 hybridoma supernatants with HeLa cell (MICA positive) lysate. The MICA-specific band is approximately 62 kDa.
